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Her3/ErbB3(Ab-1328) Antibody#21510

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Product Detail

Product NameHer3/ErbB3(Ab-1328) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Her3/ErbB3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3.

Target NameHer3/ErbB3

ConjugateUnconjugated

Other Names Tyrosine kinase-type cell surface receptor HER3

Accession NoSwiss-Prot: P21860
NCBI Protein: NP _001005915.1

Uniprot P21860

Gene ID 2065;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from A431 cells using Her3/ErbB3(Ab-1328) Antibody #21510.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Ab-1328) Antibody #21510(left) or the same antibody preincubated with blocking peptide(right).
Binds and is activated by neuregulins and NTAK.

If you have published an article using product 21510, please notify us so that we can cite your literature.

et al,Activated ErbB3 translocates to the nucleus via clathrin-independent endocytosis, which is associated with proliferating cells. In J Biol Chem on 2016 Feb 19 by Raymond Reif, Alshaimaa Adawy,et al..PMID:26719328 , (2016),
PMID: 26719328

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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