Product Detail
Product NamemTOR(Ab-2481) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total mTOR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.2479~2483 (I-H-S-F-I) derived from Human mTOR.
Target NamemTOR
ConjugateUnconjugated
Other NamesFRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein
Accession NoSwiss-Prot: P42345
NCBI Protein: NP_004949.1
Uniprot
P42345
Gene ID
2475;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 289kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR(Ab-2481) Antibody #21515(left) or the same antibody preincubated with blocking peptide(right).
Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815-822.
Brown, E.J. et al. (1994) Nature 369, 756-758.
Dennis, P.B. et al. (2001) Science 294, 1102-1105.
Sabatini, D.M. et al. (1994) Cell 78, 35-43.
If you have published an article using product 21515, please notify us so that we can cite your literature.
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