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HDAC4/HDAC5/HDAC9(Ab-246/259/220) Antibody#21517

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Product Detail

Product NameHDAC4/HDAC5/HDAC9(Ab-246/259/220) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total HDAC4/HDAC5/HDAC9 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.244~248/257~261/218~222 (T-A-S-E-P) derived from Human HDAC4/HDAC5/HDAC9.

Target NameHDAC4/HDAC5/HDAC9

ConjugateUnconjugated

Other NamesHD4/HD5/HD9

Accession NoSwiss-Prot: P56524 Q9UQL6 Q9UKV0
NCBI Protein: NP_006028.2 NP_001015053.1 NP_055522.1

Uniprot P56524

Gene ID 9759;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 140 124 111 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 and Hela cells using HDAC4/HDAC5/HDAC9(Ab-246/259/220) Antibody #21517.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9(Ab-246/259/220) Antibody #21517(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using HDAC4/HDAC5/HDAC9(Ab-246/259/220) Antibody #21517.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
Vigushin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218.
Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

If you have published an article using product 21517, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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