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b-Catenin(Ab-654) Antibody#21520

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Product Detail

Product Nameb-Catenin(Ab-654) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total b-Catenin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.652~656 (A-T-Y-A-A) derived from Human b-Catenin.

Target Nameb-Catenin

Other NamesCTNNB1; CATNB; CTNB1; CTNNB;

Accession NoSwiss-Prot: P35222
NCBI Protein: NP_001091679.1

Uniprot P35222

Gene ID 1499;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 and Hela cells using b-Catenin(Ab-654) Antibody #21520.
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

If you have published an article using product 21520, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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