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PLC-g2(Ab-1217) Antibody#21524

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Product Detail

Product NamePLC-g2(Ab-1217) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total PLC-g2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCg2.

Target NamePLC-g2

ConjugateUnconjugated

Other NamesPLC-IV; PLC-gamma2; Phospholipase C-gamma-2

Accession NoSwiss-Prot: P16885
NCBI Protein: NP_002652.2

Uniprot P16885

Gene ID 5336;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 150kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-g2(Ab-1217) Antibody #21524(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g2(Ab-1217) Antibody #21524.
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Smrcka, A. V. et al. (1991) Science 251, 804-807.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.

If you have published an article using product 21524, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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