Product Detail
Product NamePLC-g2(Ab-1217) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total PLC-g2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCg2.
Target NamePLC-g2
ConjugateUnconjugated
Other NamesPLC-IV; PLC-gamma2; Phospholipase C-gamma-2
Accession NoSwiss-Prot: P16885
NCBI Protein: NP_002652.2
Uniprot
P16885
Gene ID
5336;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 150kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-g2(Ab-1217) Antibody #21524(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g2(Ab-1217) Antibody #21524.
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Smrcka, A. V. et al. (1991) Science 251, 804-807.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.
If you have published an article using product 21524, please notify us so that we can cite your literature.