Product Detail
Product NamePaxillin(Ab-88) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Paxillin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin.
Target NamePaxillin
ConjugateUnconjugated
Other NamesPAXI; PXN;
Accession NoSwiss-Prot: P49023
NCBI Protein: NP_001074324.1
Uniprot
P49023
Gene ID
5829;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:50~1:100
Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody #21538 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) Antibody #21538.
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441
Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.
If you have published an article using product 21538, please notify us so that we can cite your literature.
et al,Lipid rafts control human melanoma cell migration by regulating focal adhesion disassembly. In Biochim Biophys Acta on 2013 Dec by Ruifei Wang, Jiajia Bi, et al..PMID: 24055995
, (2013),
PMID:
24055995
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et
al..PMID: 23665237
, (2013),
PMID:
23665237
Yes
