Product Detail
Product Namec-kit(Ab-936) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total c-Kit protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit.
Target Namec-kit
ConjugateUnconjugated
Other NamesKIT; CD117; SCFR; PBT;
Accession NoSwiss-Prot: P10721
NCBI Protein: NP_000213.1
Uniprot
P10721
Gene ID
3815;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HL60 cells using c-kit(Ab-936) Antibody #21539.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Ab-936) Antibody #21539(left) or the same antibody preincubated with blocking peptide(right).
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Martin, F.H. et al. (1990) Cell 63, 203-11.
Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
Nocka, K. et al. (1990) EMBO J 9, 1805-13.
If you have published an article using product 21539, please notify us so that we can cite your literature.
et al,Characterization of Long-Term Cultured c-kit+ Cardiac Stem Cells Derived From Adult Rat Hearts. In Stem Cells Dev on 2010 Jan by Shinka Miyamoto, Nanako Kawaguchi, et al..PMID: 19580375
, (2010),
PMID:
19580375
Yes
