Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > Cyclin B1(Ab-147) Antibody

Cyclin B1(Ab-147) Antibody#21540

Availability: Yes
Total Amount:$
Product Detail

Product NameCyclin B1(Ab-147) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total Cyclin B1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.

Target NameCyclin B1

ConjugateUnconjugated

Accession NoSwiss-Prot: P14635
NCBI Protein: NP_114172.1

Uniprot P14635

Gene ID 891;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 and HT29 cells using Cyclin B1(Ab-147) Antibody #21540.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1(Ab-147) Antibody #21540(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1(Ab-147) Antibody #21540.

The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

If you have published an article using product 21540, please notify us so that we can cite your literature.

et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762 , (2014),
PMID: 25412762

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;