Product Detail
Product NameFAK(Ab-576/577) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total FAK protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.574~578/575~579 (T-Y-Y-K-A) derived from Human FAK.
Target NameFAK
ConjugateUnconjugated
Other NamesFADK 1; FAK1; PTK2
Accession NoSwiss-Prot: Q05397
NCBI Protein: NP _005598.3
Uniprot
Q05397
Gene ID
5747;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 and Hela cells using FAK(Ab-576/577) Antibody #21545.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK(Ab-576/577) Antibody #21545(left) or the same antibody preincubated with blocking peptide(right).
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
If you have published an article using product 21545, please notify us so that we can cite your literature.
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer
, (2021),
PMID:
34830928
Yes
