Product Detail
Product Namesyk(Ab-323) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total syk protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
Target Namesyk
ConjugateUnconjugated
Other NamesSpleen tyrosine kinase
Accession NoSwiss-Prot: P43405
NCBI Protein: NP_001128524.1
Uniprot
P43405
Gene ID
6850;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from JK cells using syk(Ab-323) Antibody #21546.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk(Ab-323) Antibody #21546(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using syk(Ab-323) Antibody #21546.
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
If you have published an article using product 21546, please notify us so that we can cite your literature.
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