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Location： Home > Polyclonal Antibodies > MDM2(Ab-166) Antibody

MDM2(Ab-166) Antibody#21550

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Product Detail

Product NameMDM2(Ab-166) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt Monkey

SpecificityThe antibody detects endogenous level of total MDM2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.164~168 (A-T-S-E-T) derived from Human MDM2.

Target NameMDM2

ConjugateUnconjugated

Other NamesHDMX, hdm2

Accession NoSwiss-Prot: Q00987
NCBI Protein: NP_002383.2

Uniprot Q00987

Gene ID 4193;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29, Hela and 293 cells using MDM2(Ab-166) Antibody #21550.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Ab-166) Antibody #21550(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MDM2(Ab-166) Antibody #21550.
Western blot analysis on COS7 cell lysates using MDM2 Antibody. The lane on the left was treated with the antigen-specific peptide.
This image is a courtesy of anonymous review
21550 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.

If you have published an article using product 21550, please notify us so that we can cite your literature.

et al,Enhanced Proliferation of Bone Marrow Mesenchymal Stem Cells by Co-Culture With TM4 Mouse Sertoli Cells: Involvement of the EGF/PI3K/AKT Pathway .In Mol Cell Biochem on 2014 Aug byHuan Tian , Meijin Guo et al..PMID:24748323 , (2014),
PMID: 24748323

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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