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NMDANR2B Subunit Antibody#21560

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Product Detail

Product NameNMDANR2B Subunit Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total NMDANR2B Subunit protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.1250-1254(N-L-Y-D-I) derived from Human NMDANR2B Subunit.

Target NameNMDANR2B Subunit

ConjugateUnconjugated

Other NamesNMDAR2B; NR2B;

Accession NoSwiss-Prot: Q00960
NCBI Protein: NP_036706.1

Uniprot Q00960

Gene ID 24410;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 180kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from T98G cells using NMDANR2B Subunit Antibody #21560
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death

Kornau H.C., Schenker L.T., Kennedy M.B., Seeburg P.H.Science 269:1737-1740(1995)
Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J.Mol. Cell. Neurosci. 11:161-172(1998)

If you have published an article using product 21560, please notify us so that we can cite your literature.

et al,Bisphenol-A Induces neurodegeneration through disturbance of intracellular calcium homeostasis in human embryonic stem cells-derived cortical neurons Chemosphere. In Chemosphere on 2019 Aug by Wang H, Zhao P, et al..PMID: 31102917 , (2019),
PMID: 31102917

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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