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CK2a Antibody#21572

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Product Detail

Product NameCK2a Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total CK2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 353~357 (S-G-I-S-S) derived from Human CK2α.

Target NameCK2a

ConjugateUnconjugated

Other NamesCKII; CK2A1; CSNK2A1

Accession NoSwiss-Prot: P68400
NCBI Protein: NP_001886.1

Uniprot P68400

Gene ID 1457;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 42kd
Western blotting: 1:1000

Western blot of CK2a(Phospho-Thr360/Ser362) antibody(#11572) and CK2aantibody(#21572) in vitro kinase assay. Both purified ERK2 and CK2 were used.

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)
Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)
Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)

If you have published an article using product 21572, please notify us so that we can cite your literature.

et al,A Small Organic Compound Mimicking the L1 Cell Adhesion Molecule Promotes Functional Recovery after Spinal Cord Injury in Zebrafish.In Mol Neurobiol.On 2018 Jan by Sahu S, Zhang Z et al..PMID:28070857 , (2018),
PMID: 28070857

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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