Product Detail
Product NamePKM1 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous levels of total PKM1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 399~403(V-R-A-S-S) derived from Human PKM1.
Target NamePKM1
ConjugateUnconjugated
Other NamesPKM; PK3; OIP3; PK2;
Accession NoSwiss-Prot: P14618
NCBI Protein: NP_872270.1
Uniprot
P14618
Gene ID
5315;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:1000
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.
If you have published an article using product 21577, please notify us so that we can cite your literature.
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by
Weiwei Yang, Yan Xia, et al..PMID: 23123196
, (2012),
PMID:
23123196
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803
, (2012),
PMID:
22901803
et al,Phosphorylation of pyruvate kinase M2 and lactate dehydrogenase A by fibroblast growth factor receptor 1 in benign and malignant thyroid tissue.In BMC Cancer on 2015 Mar 18 by Paul Kachel , Bogusz Trojanowicz et al..PMID:25880801
, (2015),
PMID:
25880801
et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223
, (2014),
PMID:
24316223
et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762
, (2014),
PMID:
25412762
et al,Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling.In Cell Signal on 2014 Sep by Peng Yang, Zongwei Li et al..PMID:24686087
, (2014),
PMID:
24686087
et al,Pyruvate kinase M2 accelerates pro-inflammatory cytokine secretion and cell proliferation induced by lipopolysaccharide in colorectal cancer. In Cell Signal on 2015 Jul by Peng Yang, Zongwei Li, et al..PMID:25778902
, (2015),
PMID:
25778902
More
et al,Regulatory circuit of PKM2/NF-魏B/miR-148a/152-modulated tumor angiogenesis and cancer progression. In Oncogene on 2015 Oct by
Q Xu, L-Z Liu,et al..PMID:25703326
, (2015),
PMID: 25703326
et al,A novel miR-206/hnRNPA1/PKM2 axis reshapes the Warburg effect to suppress colon cancer growth. In Biochem Biophys Res Commun on 2020 Oct 22 by Rong Fu, Peng Yang, et al..PMID:32800545
, (2020),
PMID: 32800545
et al,Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation. In Biomedicines on 2022 May 27 by Abu Jubayer Hossain, Rokibul Islam, et al..PMID:35740278
, (2022),
PMID: 35740278
et al,Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition. In Biomolecules on 2022 Aug 6 by Seong Ho Kim, Ji Hun Wi, et al..PMID:36008976
, (2022),
PMID: 36008976
et al,Function of a complex of p-Y42 RhoA GTPase and pyruvate kinase M2 in EGF signaling pathway in glioma cells.
, (2025),
PMID: 39183510
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