Product Detail
Product Namea-Tubulin Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total alpha-Tubulin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.437-441(V-D-S-V-E) derived from Human alpha-Tubulin.
Target Namea-Tubulin
ConjugateUnconjugated
Other NamesK-ALPHA-1
Accession NoSwiss-Prot: P68363
NCBI Protein: NP_006073.2
Uniprot
P68363
Gene ID
10376;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and HT29 cells using alpha-Tubulin Antibody #21581.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain.
Cowan N.J., Dobner P., Fuchs E.V., Cleveland D.W.Mol. Cell. Biol. 3:1738-1745(1983)
If you have published an article using product 21581, please notify us so that we can cite your literature.
et al,Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Prevents Apoptosis Induced by Hydrogen Peroxide in Basilar Artery Smooth Muscle Cells .In Apoptosis On 2014 Sep by Jia-Wei Zeng , Xue-Lin Zeng et al..PMID:24999019
, (2014),
PMID:
24999019
et al,Multifunctional manganese-based nanogels catalyze immune energy metabolism to promote bone repair
, (2025),
PMID:
et al,Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5'tRFAla/DVL/β-catenin pathway inhibition.
, (2025),
PMID:
40128187
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