Product Detail
Product NameTubulin b-III Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total Tubulin b-III protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.442~446(E-E-S-E-A) derived from Human Tubulin b-III.
Target NameTubulin b-III
ConjugateUnconjugated
Other NamesTUBB4; CFEOM3A; TUBB3
Accession NoSwiss-Prot: Q13509
NCBI Protein: NP_006077.2
Uniprot
Q13509
Gene ID
10381;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 55kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Mouse brain tissue, Hela, JK and P19 cells using Tubulin beta-III Antibody #21617
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Tischfield M.A., Baris H.N., Wu C., Rudolph G. Cell 140:74-87(2010)
Katsetos C.D., Legido A., Perentes E., Mork S.J. J. Child Neurol. 18:851-866(2003)
If you have published an article using product 21617, please notify us so that we can cite your literature.
et al,Proteostatic modulation in brain aging without associated Alzheimer's disease-and age-related neuropathological changesInAgingOn2023 May 13byPol Andrés-Benito?1?2,?Ignacio í?igo-Marco et al..PMID:?37179123
, (2023),
PMID:
37179123
et al,PQBP1 regulates striatum development through balancing striatal progenitor proliferation and differentiationIn Cell RepOn2023 Mar 28byWenhua Liu , Hao Xie et al..PMID:36943865
, (2023),
PMID:
36943865
Yes
