Product Detail
Product NameLIN28B Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous levels of total LIN28B protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
Target NameLIN28B
ConjugateUnconjugated
Other NamesCSDD2; FLJ16517;
Accession NoSwiss-Prot: Q6ZN17
NCBI Protein: NP_001004317.1
Uniprot
Q6ZN17
Gene ID
389421;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 21 32kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and 293 cells using LIN28B Antibody #21626.
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. "Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma."
Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA."
Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation."
Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009)
If you have published an article using product 21626, please notify us so that we can cite your literature.
et al,Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics.In Mol Med Rep on 2020 May;21 by Wang B, Wang L, et al..PMID: 32186775
, (2020),
PMID:
32186775
et al,Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway.In Mol Med Rep.On 2016 Oct by Gu H, Ji R et al..PMID:27513187
, (2016),
PMID:
27513187
et al,The KRAS/Lin28B axis maintains stemness of pancreatic cancer cells via the let锟?i/TET3 pathway. In Mol Oncol on 2021 Jan by Yawen Liu, Dawei Wang,et al..PMID:33107691
, (2021),
PMID:
33107691
Yes
