Product Detail
Product NameRing1A Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Ring1A protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.35~39( I-A-V-S-P) derived from Human Ring1A.
Target NameRing1A
ConjugateUnconjugated
Other NamesRNF1; RING1;
Accession NoSwiss-Prot: Q06587
NCBI Protein: NP_002922.2
Uniprot
Q06587
Gene ID
6015;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 58kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Hela cells using Ring1A Antibody #21629
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Cao R., Tsukada Y., Zhang Y.Mol. Cell 20:845-854(2005)
Satijn D.P.E., Gunster M.J., van der Vlag J., Hamer K.M. Mol. Cell. Biol. 17:4105-4113(1997)
If you have published an article using product 21629, please notify us so that we can cite your literature.
Yes
