Product Detail
Product NameDoublecortin Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Doublecortin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.413~417( P-T-S-P-G) derived from Human Doublecortin.
Target NameDoublecortin
ConjugateUnconjugated
Other NamesDCX; DC; DBCN
Accession NoSwiss-Prot: O43602
NCBI Protein: NP_000546.2
Uniprot
O43602
Gene ID
1641;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 40-45kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Molt-4 and Hela cells using Doublecortin Antibody #21630
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
Des Portes V., Pinard J.-M., Billuart P. Chelly J.Cell 92:51-61(1998)
Pilz D.T., Matsumoto N., Minnerath S.R., Mills P. Hum. Mol. Genet. 7:2029-2037(1998)
If you have published an article using product 21630, please notify us so that we can cite your literature.
Yes
