Product Detail
Product NameWASP Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total WASP protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.289~293 (L-I-Y-D-F) derived from Human WASP.
Target NameWASP
ConjugateUnconjugated
Other NamesTHC; THC1; IMD2
Accession NoSwiss-Prot: P42768
NCBI Protein: NP_000368.1
Uniprot
P42768
Gene ID
7454;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Hela, JK and Raji cells using WASP Antibody #21632
Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.
Kolluri R., Tolias K.F., Carpenter C.L., Rosen F.S. Proc. Natl. Acad. Sci. U.S.A. 93:5615-5618(1996)
Ramesh N., Anton I.M., Hartwig J.H., Geha R.S. Proc. Natl. Acad. Sci. U.S.A. 94:14671-14676(1997)
Cory G.O.C., Cramer R., Blanchoin L., Ridley A.J. Mol. Cell 11:1229-1239(2003)
If you have published an article using product 21632, please notify us so that we can cite your literature.
Yes
