Product Detail
Product NamePPARg Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous levels of total PPARg protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.50~54 (L-S-V-M-E) derived from Human PPARg
Target NamePPARg
ConjugateUnconjugated
Other NamesGLM1; NR1C3; PPARG1; PPARG2; PPARG
Accession NoSwiss-Prot: P37231
NCBI Protein: NP_005028.4
Uniprot
P37231
Gene ID
5468;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 53 57kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela, 293, 3T3 and MCF-7 cells using PPARg Antibody #21649.
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. "Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARgamma2 versus PPARgamma1 and activation with retinoid X receptor agonists and antagonists."
Mukherjee R., Jow L., Croston G.E., et al. J. Biol. Chem. 272:8071-8076(1997) "Cloning and characterization of RAP250, a nuclear receptor coactivator."
Caira F., Antonson P., Pelto-Huikko M., et al. J. Biol. Chem. 275:5308-5317(2000) "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor a."
Qi C., Chang J., Zhu Y.,et al. J. Biol. Chem. 277:28624-28630(2002)
If you have published an article using product 21649, please notify us so that we can cite your literature.
et al,Adropin Contributes to Anti-Atherosclerosis by Suppressing Monocyte-Endothelial Cell Adhesion and Smooth Muscle Cell Proliferation.In Int J Mol Sci. On 2018 Apr 26 by Sato K, Yamashita T et al..PMID: 29701665
, (2018),
PMID:
29701665
et al,β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques.In Int J Endocrinol on 2020 Mar 28; by Okano T, Sato K,et al..PMID:32308678
, (2020),
PMID:
32308678
Yes
