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NAC1 Antibody#21652

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Product Detail

Product NameNAC1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total NAC1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.519~523(A-G-P-S-A) derived from Human NAC1

Target NameNAC1

ConjugateUnconjugated

Other Names BEND8; NACC1; NAC-1; BTBD14B; FLJ37383

Accession NoSwiss-Prot: Q96RE7
NCBI Protein: NP_443108.1

Uniprot Q96RE7

Gene ID 112939;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 62kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Rat and Mouse brain tissue using NAC1 Antibody #21652.

Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines. "A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival."
Nakayama K., Nakayama N., Davidson B.,et al.
Proc. Natl. Acad. Sci. U.S.A. 103:18739-18744(2006) "NAC-1 controls cell growth and survival by repressing transcription of Gadd45GIP1, a candidate tumor suppressor."
Nakayama K., Nakayama N., Wang T.-L., Shih I.-M. Cancer Res. 67:8058-8064(2007)

If you have published an article using product 21652, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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