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HNF-1b(TCF-2) Antibody#21658

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Product Detail

Product NameHNF-1b(TCF-2) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total HNF-1b(TCF-2) protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.252~256(R-Q-K-N-P) derived from Human HNF-1b(TCF-2).

Target NameHNF-1b(TCF-2)

ConjugateUnconjugated

Other NamesFJHN; HNF2; LFB3; TCF2; HPC11

Accession NoSwiss-Prot: P35680
NCBI Protein: NP_001159395.1

Uniprot P35680

Gene ID 6928;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 45,58,61kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from Hela, K562 and 3T3 cells using HNF-1b(TCF-2) Antibody #21658.

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Lindner T.H., Njoelstad P.R., Horikawa Y., Bostad L. Mol. Genet. 8:2001-2008(1999)
Weng J.P., Lehto M., Forsblom C., Huang X. Diabetologia 43:131-132(2000)

If you have published an article using product 21658, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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