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HDAC3 Antibody#21660

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Product Detail

Product NameHDAC3 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total HDAC3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.420~424(N-D-K-E-S) derived from Human HDAC3.

Target NameHDAC3

ConjugateUnconjugated

Other NamesHD3; RPD3; RPD3-2

Accession NoSwiss-Prot: O15379
NCBI Protein: NP_003874.2

Uniprot O15379

Gene ID 8841;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 49kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from Hela, K562 and 3T3 cells using HDAC3 Antibody #21660.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation

Wei L.-N., Hu X., Chandra D., Seto E., Farooqui M.J. Biol.Chem. 275:40782-40787(2000)
Li H., Leo C., Zhu J., Wu X., O'Neil J., Park E.-J., Chen J.D.Mol. Cell. Biol. 20:1784-1796(2000)

If you have published an article using product 21660, please notify us so that we can cite your literature.

et al,Growth Attenuation Is Associated With Histone Deacetylase 10-induced Autophagy in the Liver .In J Nutr Biochem on 2016 Jan by Galit Pinto, Biana Shtaif et al..PMID:26462881 , (2016),
PMID: 26462881

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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