Product Detail
Product NameC-Fos Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total C-Fos protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.28~32( S-Y-Y-H-S) derived from Human C-Fos.
Target NameC-Fos
ConjugateUnconjugated
Other Namesp55; AP-1;
Accession NoSwiss-Prot: P01100
NCBI Protein: NP_005243.1
Uniprot
P01100
Gene ID
2353;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 62kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from A549, Hela and 3T3 cells using C-Fos Antibody #21667.
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Okazaki K., Sagata N.EMBO J. 14:5048-5059(1995)
Zhang Y., Feng X.H., Derynck R.Nature 394:909-913(1998)
Bossis G., Malnou C.E., Farras R. Mol. Cell. Biol. 25:6964-6979(2005)
If you have published an article using product 21667, please notify us so that we can cite your literature.
et al,Piperine alleviates osteoclast formation through the p38/c鈥怓os/NFATc1 signaling axis.In Biofactors on Nov-Dec 2015 by Vishwa Deepak , Marlena C Kruger et al..PMID: 26627060
, (2015),
PMID:
26627060
Yes
