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IGFBP-3 Antibody#21671

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Product Detail

Product NameIGFBP-3 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total IGFBP-3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.185~189(R-Y-K-V-D) derived from Human IGFBP-3.

Target NameIGFBP-3

ConjugateUnconjugated

Other NamesIBP3; BP-53;

Accession NoSwiss-Prot: P17936
NCBI Protein: NP_001013416.1

Uniprot P17936

Gene ID 3486;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 24-41kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from various cells using IGFBP-3 Antibody #21671.

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Roghani M., Segovia B., Whitechurch O., Binoux M.Growth Regul. 1:125-130(1991)
Ikonen M., Liu B., Hashimoto Y., Ma L. Cohen P.Proc. Natl. Acad. Sci. U.S.A. 100:13042-13047(2003)
Zhou W., Ross M.M., Tessitore A. J. Proteome Res. 8:5523-5531(2009)

If you have published an article using product 21671, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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