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PCNA Mouse Monoclonal Antibody#21801

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Product Detail

Product NamePCNA Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHu Ms Rt

Immunogen TypePeptide-KLH

Immunogen DescMouse monoclonal antibody is prepared by immunizing a synthetic peptide.

Target NamePCNA

ConjugateUnconjugated

Other NamesCyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen.

Accession NoSwiss-Prot:P12004
Gene ID:5111

Uniprot P12004

Gene ID 5111;

Sdspage MW29KD

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C/1 year

Application Details
 WB: 1:3000~1:10000   
 IHC: 1:200

 

Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.

 PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

If you have published an article using product 21801, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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