Product Detail
Product NameASS1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 155 and 410 of Human ASS1
Target NameASS1
ConjugateUnconjugated
Accession NoSwiss-Prot:P00966
Gene ID:445
Uniprot
P00966
Gene ID
445;
Concentration0.7mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 47kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30 ug of whole cell lysate)
A: A431
12% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded mixed OVCA, using ASS1 antibody at 1: 100 dilution.
Immunofluorescence analysis of methanol-fixed HCT116, using ASS1 antibody at 1: 500 dilution.
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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