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MAF1 antibody#22052

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Product Detail

Product NameMAF1 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IF

Species ReactivityHu

Immunogen TypeRecombinant protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 171 of MAF1

Target NameMAF1

ConjugateUnconjugated

Accession NoSwiss-Prot:Q9H063
Gene ID:84232

Uniprot Q9H063

Gene ID 84232;

Concentration1mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
Predicted MW: 29kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: A431
B: MOLT4
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1: 200 dilution.
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

If you have published an article using product 22052, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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