Product Detail
Product Namenucleoporin p62 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 230 and 500 of nucleoporin p62
Target Namenucleoporin p62
ConjugateUnconjugated
Accession NoSwiss-Prot:P37198
Gene ID:23636
Uniprot
P37198
Gene ID
23636;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: Hep G2
B: MOLT4
C: Raji
10% SDS PAGE
Primary antibody diluted at 1: 5000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 antibody at 1: 500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Nucleoporin p62 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]
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