Product Detail
Product NameERCC6 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 346 and 736 of ERCC6
Target NameERCC6
ConjugateUnconjugated
Accession NoSwiss-Prot:Q03468|P0DP91
Gene ID:2074
Uniprot
P0DP91
Gene ID
2074;
Concentration0.3mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 168kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: A431
B: H1299
7.5% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded OVCA, using CSB antibody at 1: 250 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC6 antibody at 1: 200 dilution.
This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity; there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B. [provided by RefSeq]
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