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RAG2 antibody#22074

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Product Detail

Product NameRAG2 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypeRecombinant protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 271 and 519 of RAG2

Target NameRAG2

ConjugateUnconjugated

Accession NoSwiss-Prot:P55895
Gene ID:5897

Uniprot P55895

Gene ID 5897;

Concentration0.4mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 59kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
 

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2 antibody at 1: 200 dilution.

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]

If you have published an article using product 22074, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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