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ST3GAL1 antibody#22094

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Product Detail

Product NameST3GAL1 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 165 of Human ST3GAL1

Target NameST3GAL1

ConjugateUnconjugated

Other NamesGal-NAc6S

Accession NoSwiss-Prot:Q11201
Gene ID:6482

Uniprot Q11201

Gene ID 6482;

Concentration0.63mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 39kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200

Sample(30 ug of whole cell lysate)
A: HeLa S3
12% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded serous OVCA, using ST3GAL1 antibody at 1: 100 dilution.
Immunofluorescence analysis of methanol-fixed Hep3B, using SIAT4A antibody at 1: 500 dilution.

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq]

If you have published an article using product 22094, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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