Product Detail
Product NamePeflin antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 77 and 265 of Peflin
Target NamePeflin
ConjugateUnconjugated
Accession NoSwiss-Prot:Q9UBV8
Gene ID:553115
Uniprot
Q9UBV8
Gene ID
553115;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 30kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: 293T
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin antibody at 1: 200 dilution.
PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]
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