Product Detail
Product NameNDUFS2 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 200 and 400 of NDUFS2
Target NameNDUFS2
ConjugateUnconjugated
Accession NoSwiss-Prot:O75306
Gene ID:4720
Uniprot
O75306
Gene ID
4720;
Concentration0.9mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS2 antibody at 1: 250 dilution.
Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]
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Yes
