Product Detail
Product NameTAP antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAffinity purified by Protein A.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 449 and 598 of TAP
Target NameTAP
ConjugateUnconjugated
Accession NoSwiss-Prot:Q9UBU9
Gene ID:10482
Uniprot
Q9UBU9
Gene ID
10482;
Concentration10mg/ml
FormulationSupplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 70kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1: 200 dilution.
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
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