Product Detail
Product NameGOLGA3 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypePeptide
Immunogen DescSynthetic peptide contain a sequence corresponding to a region within amino acids 1436 and 1498 of Golgi autoantigen, golgin subfamily a, 3
Target NameGOLGA3
ConjugateUnconjugated
Accession NoSwiss-Prot:Q08378
Gene ID:2802
Uniprot
Q08378
Gene ID
2802;
Concentration1mg/ml
FormulationSupplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 167kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30 ug of whole cell lysate)
A: A431
B: H1299
5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded gastric tissue, using GOLGA3 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GOLGA3 antibody at 1: 200 dilution.
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq]
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