Product Detail
Product NameAKR1C1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 22 and 218 of Human AKR1C1
Target NameAKR1C1
ConjugateUnconjugated
Accession NoSwiss-Prot:Q04828
Gene ID:1645
Uniprot
Q04828
Gene ID
1645;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 37kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: Raji
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using AKR1C1 antibody at 1: 500 dilution.
Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody at 1: 200 dilution.
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]
If you have published an article using product 22149, please notify us so that we can cite your literature.
Yes
