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PPA1 antibody#22152

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Product Detail

Product NamePPA1 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPurified by antigen-affinity chromatography.

ApplicationsWB IHC IF

Species ReactivityHu

Immunogen TypeRecombinant protein

Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 259 of PPA1

Target NamePPA1

ConjugateUnconjugated

Accession NoSwiss-Prot:Q15181
Gene ID:5464

Uniprot Q15181

Gene ID 5464;

Concentration0.9mg/ml

FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

Predicted MW: 33kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
D: Molt-4
12% SDS PAGE
Primary antibody diluted at 1: 10000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PPA1 antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1 antibody at 1: 200 dilution.

The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq]

If you have published an article using product 22152, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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