Product Detail
Product NameCKAP1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 213 of Human TBCB
Target NameCKAP1
ConjugateUnconjugated
Accession NoSwiss-Prot:Q99426
Gene ID:1155
Uniprot
Q99426
Gene ID
1155;
Concentration0.4mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 27kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using CKAP1 antibody at 1: 500 dilution.
Immunofluorescence analysis of methanol-fixed A549, using CKAP1 antibody at 1: 500 dilution.
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
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