Product Detail
Product Nameglyoxalase I antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 171 of glyoxalase I
Target Nameglyoxalase I
ConjugateUnconjugated
Accession NoSwiss-Prot:Q04760
Gene ID:2739
Uniprot
Q04760
Gene ID
2739;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 21kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample(30ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded OVCA, using GLO1 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using glyoxalase I antibody at 1: 200 dilution.
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]
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