Product Detail
Product NameGCP2 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 196 of Human TUBGCP2
Target NameGCP2
ConjugateUnconjugated
Accession NoSwiss-Prot:Q9BSJ2
Gene ID:10844
Uniprot
Q9BSJ2
Gene ID
10844;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 103kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: A431
B: Hela
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GCP2 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
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