Product Detail
Product NamePOMGNT1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 98 and 343 of Human POMGNT1
Target NamePOMGNT1
ConjugateUnconjugated
Accession NoNCBI Gene ID: 55624
NCBI mRNA#: NM_017739
NCBI Protein#: NP_060209
Uniprot
Q8WZA1
Gene ID
55624;
Concentration0.9mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 75kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using POMGNT1 antibody at 1: 500 dilution.
Immunofluorescence analysis of methanol-fixed Hep3B, using POMGNT1 antibody at 1: 500 dilution.
The protein encoded by this gene is a type II transmembrane protein that resides in the golgi. It participates in O-mannosyl glycosylation, and is specific for alpha linked terminal mannose. Mutations in this gene are associated with muscle-eye-brain (MEB) disease. Alternatively spliced transcript variants have been found for this gene.
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