Product Detail
Product NamePhosphomannomutase 2 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 174 (O15305) of PMM2
Target NamePhosphomannomutase 2
ConjugateUnconjugated
Accession NoSwiss-Prot:O15305
Gene ID:5373
Uniprot
O15305
Gene ID
5373;
Concentration0.2mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 28kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PMM2 antibody at 1: 100 dilution.
The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq]
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