Product Detail
Product NameCOX5B antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAffinity purified by Protein A.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 122 of Human COX5B
Target NameCOX5B
ConjugateUnconjugated
Accession NoSwiss-Prot:P10606
Gene ID:1329
Uniprot
P10606
Gene ID
1329;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 14kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Sample(30 ug of whole cell lysate)
A: Hep G2
15% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using COX5B antibody at 1: 100 dilution.
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq]
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