Product Detail
Product NameRadixin antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 95 and 444 of Radixin
Target NameRadixin
ConjugateUnconjugated
Other NamesDFNB24
Accession NoSwiss-Prot:P35241
Gene ID:5962
Uniprot
P35241
Gene ID
5962;
Concentration0.5mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 69kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
Primary antibody diluted at 1: 5000
A:Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Radixin antibody at 1: 500 dilution. B:Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Radixin antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Radixin antibody at 1: 200 dilution.
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. [provided by RefSeq]
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