Product Detail
Product NameHLA-DMB antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypePeptide
Immunogen DescSynthetic peptide contain a sequence corresponding to a region within amino acids 199 and 263 of Human HLA-DMB
Target NameHLA-DMB
ConjugateUnconjugated
Other NamesRING7; D6S221E
Accession NoSwiss-Prot:P28068
Gene ID:3109
Uniprot
P28068
Gene ID
3109;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 29kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody.
A: Non-transfected lysate.
B: HLA-DMB transfected lysate.
12% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded Colon ca, using HLA-DMB antibody at 1: 250 dilution.
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq]
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