Product Detail
Product NameEnolase 3 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 196 and 413 of human ENO3
Target NameEnolase 3
ConjugateUnconjugated
Accession NoSwiss-Prot:P13929
Gene ID:2027
Uniprot
P13929
Gene ID
2027;
Concentration0.5mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 47kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
C: Raji
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ENO3 antibody at 1: 500 dilution.
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR. [provided by RefSeq]
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