Product Detail
Product NameUFD1L antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IHC IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 277 of UFD1L
Target NameUFD1L
ConjugateUnconjugated
Accession NoSwiss-Prot:Q92890
Gene ID:7353
Uniprot
Q92890
Gene ID
7353;
Concentration1mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 35kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGEPrimary antibody diluted at 1: 10000
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L antibody at 1: 200 dilution.
The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, that is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
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