Product Detail
Product NameFlightless I antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPurified by antigen-affinity chromatography.
ApplicationsWB IF
Species ReactivityHu
Immunogen TypeRecombinant protein
Immunogen DescRecombinant protein fragment contain a sequence corresponding to a region within amino acids 435 and 748 of Flightless I
Target NameFlightless I
ConjugateUnconjugated
Accession NoSwiss-Prot:Q13045
Gene ID:2314
Uniprot
Q13045
Gene ID
2314;
Concentration0.9mg/ml
FormulationSupplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details
Predicted MW: 145kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: Raji
5% SDS PAGE
Primary antibody diluted at 1: 1000
A:Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1: 200 dilution. B:Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]
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